The separation of the histamine-sensitizing factor from the protective antigens of Bordetella pertussis.

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  • 1 October 1958
    • journal article
    • Vol. 1  (4) , 328-37
Abstract
The histamine-sensitizing factor of Bordetella pertussis was shown by fractionation of extracts to be different from the antigen protecting mice against an intracerebral challenge. Ten per cent methanol precipitates from a calcium chloride extract of broken cells a water-soluble fraction containing the histamine-sensitizing factor and the antigen protecting mice against an intracerebral challenge. Dialysis of this fraction yields a precipitate soluble at pH7, containing a much greater amount of protective antigen than histamine sensitizing factor and supernatant which has high histamine sensitizing activity and is of poor immunizing potency.