PURIFICATION OF GONADOTROPHINS DERIVED FROM URINE AND PITUITARY GLANDS OFHUMAN BEINGS: OBSERVATIONS ON THEIR ELECTROPHORETIC BEHAVIOR AND BIOLOGICAL ACTIVITY1

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Abstract
A method for purifying human urinary gonadotrophins which is also applicable to purification of human pituitary gonadotrophins is described. Ninety-five to ninety-seven percent of the inactive impurities and toxic substances were eliminated without detectable loss of gonadotrophic activity. Bloassay of the purified products in immature hypophysectomized male and female rats indicated the presence of the three gonadotrophic activities. Electrophoretic analysis at pH 8.6 of both the urinary and pituitary preparations revealed three components migrating as anions. Electrophoretic fractionation and bioassay of the fractions on immature hypophysectomized male and female rats failed to demonstrate that each of the three gonadotrophic activities (FSH, ICSH, and luteotrophin) was associated with a different electrophoretic component, and indicated that all three of them may be associated with the central component. This evidence suggests that the human urinary and pituitary gonadotrophins are similar to each other and that either they are high molecular weight protein-like substances or that they are associated with such substances.