Enhancement of the Affinity of Nucleoli and Chromosomes for Zinc by Treatment with Chromic Acid

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Abstract
Cultured mammalian cells and wet touch preparations from human organs were fixed for 10 min. in 5;85:10 acetic-alcohol-formalin; placed in 5% aqueous CrO3 for 30 min. at 22-25 C; washed in running water 1 min.; placed in 2 mM zinc acetate in 0.14 M veronal-acetate buffer, pH 6.5, at 37 C, 30 mm; rinsed 5 sec. in 50% acetone; and stained 10 min. in a solution dithizone. This results in selective staining of the nucleoli of interphase cells, and of the chromosomes of mitotic cells.