Hibiscus latent ringspot virus, a newly recognised virus from Hibiscus rosa‐sinensis (Malvaceae) in western Nigeria

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Abstract
SUMMARY: Hibiscus latent ringspot virus (HLRV) was prevalent in Hibiscus rosa‐sinensis in Ibadan, Nigeria. It was readily transmitted mechanically to 22 of 73 species from seven of 20 families, but was best propagated in Nicotiana clevelandii or Hibiscus cannabinus and assayed in Chenopodium murale. HLRV was readily purified from systemically infected hosts by differential centrifugation of leaf extracts clarified with 8.0% n‐butanol, followed by molecular permeation chromatography on controlled‐pore glass beads (700 Å, 120–200 mesh). The virus has isometric particles c. 28 nm in diameter which sedimented as three components (T, M and B), with sedimentation coefficients (s°20, w) of 51; 114 and 132 S and buoyant densities in caesium chloride of 1.32, 1.49 and 1.52 g/cm3, respectively. All three components contained a single polypeptide of rnol. wt 53.6 × 103. T component particles contained only protein but M and B components also contained single‐stranded RNA of rnol. wt 2.2 × 106 and 2.5 × 106, respectively. The properties of HLRV suggest affinities with nepoviruses but no serological relationship was detected between HLRV and 15 recognised or possible members of the nepovirus group.