Arabidopsis consensus intron sequences
- 1 November 1996
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 32 (3) , 531-535
- https://doi.org/10.1007/bf00019105
Abstract
We have analysed 998 Arabidopsis intron sequences in the EMBL database. All Arabidopsis introns to adhere to the :GU...AG: rule with the exception of 1% of introns with :GC at their 5′ ends. Virtually all of the introns contained a putative branchpoint sequence (YUNAN) 18 to 60 nt upstream of the 3′ splice site. Although a polypyrimidine tract was much less apparent than in vertebrate introns, the most common nucleotide in the region upstream of the 3′ splice site was uridine. Consensus sequences for 5′ and 3′ splice sites and branchpoint sequences for Arabidopsis introns are presented.Keywords
This publication has 22 references indexed in Scilit:
- Mapping of branchpoint nucleotides in mutant pre‐mRNAs expressed in plant cellsThe Plant Journal, 1996
- Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes.Plant Cell, 1995
- Nuclear Pre-mRna Processing in Higher PlantsProgress in Nucleic Acid Research and Molecular Biology, 1994
- Efficient splicing of an AU-rich antisense intron sequencePlant Molecular Biology, 1993
- Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway.Plant Cell, 1992
- Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA SplicingAnnual Review of Cell Biology, 1991
- The SV40 small t intron is accurately and efficiently spliced in tobacco cellsPlant Molecular Biology, 1991
- The minimum functional length of pre-mRNA introns in monocots and dicotsPlant Molecular Biology, 1990
- Scanning from an independently specified branch point defines the 3′ splice site of mammalian intronsNature, 1989
- The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicingCell, 1989