Detection of Specific Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus Nucleocapsid Protein for Serodiagnosis of SARS Coronavirus Pneumonia
Open Access
- 1 May 2004
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (5) , 2306-2309
- https://doi.org/10.1128/jcm.42.5.2306-2309.2004
Abstract
We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 94.3, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.Keywords
This publication has 25 references indexed in Scilit:
- Diagnosis of Severe Acute Respiratory Syndrome (SARS) by Detection of SARS Coronavirus Nucleocapsid Antibodies in an Antigen-Capturing Enzyme-Linked Immunosorbent AssayJournal of Clinical Microbiology, 2003
- Isolation and Characterization of Viruses Related to the SARS Coronavirus from Animals in Southern ChinaScience, 2003
- Evaluation of Reverse Transcription-PCR Assays for Rapid Diagnosis of Severe Acute Respiratory Syndrome Associated with a Novel CoronavirusJournal of Clinical Microbiology, 2003
- The Genome Sequence of the SARS-Associated CoronavirusScience, 2003
- Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory SyndromeScience, 2003
- A Major Outbreak of Severe Acute Respiratory Syndrome in Hong KongNew England Journal of Medicine, 2003
- A Cluster of Cases of Severe Acute Respiratory Syndrome in Hong KongNew England Journal of Medicine, 2003
- Identification of Severe Acute Respiratory Syndrome in CanadaNew England Journal of Medicine, 2003
- AFLMP1 Encodes an Antigenic Cell Wall Protein in Aspergillus flavusJournal of Clinical Microbiology, 2003
- Detection of Antibodies Specific to an Antigenic Cell Wall Galactomannoprotein for Serodiagnosis of Aspergillus fumigatus AspergillosisJournal of Clinical Microbiology, 2002