Reversed-Phase High Performance Liquid Chromatography of Underivatized Fatty Acids by Fatty ACID Analysis Column

Publisher Website
Google Scholar
Abstract
Underivatized fatty acids of mammalian source after group separation by thin layer chromatography were further separated among saturates and monounsaturates as a group, and polyunsaturates as another group by reversed phase μ Bondapak Fatty Acid Analysis Column. The mobile phases consisted of tetrahydrofuran acetonitrile- water-acetic acid in ratios of 25:35:X:Y (v/v/v/v). When saturates (from C12 through C24) and monounsaturates (from C14:1 through C24:l) were analyzed, X=50 and Y = 0.2 were the best combination: When polyunsaturates (from C18:2 through C22:6) were chromatographed, X = 70 and Y = 03 provided the best resolution. Saturates were monitored by refractive index (RI) at 4X aad UV 213 nm. Monoenoic acids were scanned at UV 207nm. Potyenoic fatty acids were analyzed at UV 210 run and 235 nm. The 235 nm was for detection of possible peroxidation of those polyenoic acids. The minimal detection levels for saturates, monoenes, and polyenes were about 20 μg, 2μg, and 0.2μg at 0.01 aufs.

This publication has 15 references indexed in Scilit: