Separation of oestrogens in urinary extracts by partition chromatography
- 1 February 1955
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 59 (2) , 294-300
- https://doi.org/10.1042/bj0590294
Abstract
A method for separation and purification of estriol, estrone and estradiol 17 beta in extracts of hydrolyzed urine is described. Partition chromatography is used for the purification of estriol and for the separation and purification of estrone and estradiol-17 beta. Principal factors affecting elution of estrogens from the partition chromatograms are described in full.Keywords
This publication has 9 references indexed in Scilit:
- Some errors in the colorimetric estimation of oestriol, oestrone and oestradiol by the Kober reactionBiochemical Journal, 1954
- FRACTIONATION BY COUNTERCURRENT DISTRIBUTION OF URINARY ESTROGENS IN NORMAL INDIVIDUALS AND IN PATIENTS WITH HYPERADRENOCORTICISM*Journal of Clinical Endocrinology & Metabolism, 1953
- Chromatographic Separation of Estrone, Estradiol, and EstriolScience, 1953
- FLUORESCENCE AND ABSORPTION SPECTRA OF ESTROGENS HEATED IN SULFURIC ACIDJournal of Biological Chemistry, 1953
- The polarographic estimation of steroid hormones. 5. Determination of progesterone in bloodBiochemical Journal, 1951
- THE SEPARATION OF NATURAL ESTROGENS BY COUNTER-CURRENT DISTRIBUTIONJournal of Biological Chemistry, 1950
- The separation of the C12-C18 fatty acids by reversed-phase partition chromatographyBiochemical Journal, 1950
- THE PREPARATION OF BETA-DIHYDROEQUILIN1950
- A new form of chromatogram employing two liquid phasesBiochemical Journal, 1941