Metabolic Responses of Bovine Spermatozoa to Antibacterial Agents

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Abstract
Sulfanilamide, streptomycin, and penicillin each depressed the utilization of total reducing substances (glucose) and fructose and the production of lactic acid by bovine spermatozoa in egg yolk-citrate diluents at 4 and 10 days of storage at 4.5[degree]C. Sulfanilamide gave the most marked effects. A combination of streptomycin and penicillin (500 units of each/ml, diluted semen) depressed spermatozoan metabolism almost as much as sulfanilamide (300 [mu]g. per 100 ml. diluted semen) and gave the best livability in comparison with the other 5 diluents studied. Some of the metabolic depression by sulfanilamide is due to lowered sperm livability.