TRANSFORMATION OF HUMAN-CELLS BY DNA TRANSFECTION
- 1 January 1984
- journal article
- research article
- Vol. 44 (7) , 2769-2772
Abstract
Normal human cells can be transformed to anchorage-independent growth by transfection with DNA from MOLT-4 lymphoblasts, derived from a patient with acute lymphocytic leukemia. Cells were treated with polyethylene glycol 6000, and DNA was administered as a calcium phosphate coprecipitate. The transforming activity of MOLT DNA was inactivated by treatment with DNase or by the restriction enzymes Bg/l or BamHl but not by EcoRV. DNA from transfected colonies can transform human recipient cells to anchorage-independent growth; the transforming ability and anchorage independence are maintained stably for at least 30 generations.This publication has 11 references indexed in Scilit:
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