PREPARATION AND SERIC FORM OF RABBIT C-REACTIVE PROTEIN

Google Scholar
  • 1 January 1979
    • journal article
    • research article
    • Vol. 61  (11-1) , 1293-1299
Abstract
The preparation of rabbit C-reactive protein (CRP) involves a single step affinity chromatography. This preparation takes advantage of the Ca-dependent affinity of CRP for an agarose gel bearing 2-aminoethanol dihydrogen-phosphate as a ligand. A prior chromatography on agarose gel without the ligand allows the uptake of the serum amyloid P-component (SAP). The CRP prepared according to this method is able to form precipitating complexes in agarose with rabbit lipoproteins. The specificity of these interactions was studied. CRP-high density lipoprotein association produces a 2nd precipitation arc when the pure CRP is revealed by a specific antiserum in agarose. CRP in the serum is in the bound form only, and the binding involves low density lipoproteins exclusively.