Determination of Xylitol in the Human Serum and Saliva by Ion Chromatography with Pulsed Amperometric Detection

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Abstract
Pulsed amperometric detection with an ion chromatograph was used for the determination of xylitol in the human serum and saliva. Xylitol in serum and saliva was determined by using 0.15M sodium hydroxide as an eluent and D-mannitol as an internal standard. Xylitol and D-mannitol could be separated completely with retention times of 1.98min and 2.47min, respectively. Linearity of peak heights was 0.025 to 1.0μg/ml xylitol.The detection limit was 0.01μg/ml with an S/N=2. Mean recovery of xylitol ranged from 99.5 to 101.4%.Maximum concentrations of xylitol in serum and saliva were observed approximately 30min and 150min, respectively, after oral administration.

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