Effect of Lyophilization and Chloroform Extraction on Electrophoretic Migration of Serum Lipoproteins.

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Abstract
Paper electrophoresis of lyophilized sera showed that lyophilization markedly retards the migration of some of the cholesterol in the lipoproteins associated with the beta-globulin area although it exerts little effect on the migration of cholesterol associated with the albumin, alpha1-globulin area. The cholesterol extracted by cold chloroform from lyophilized sera came principally from the beta-globulin area or from the area around the starting point. It is suggested that the cholesterol thus extracted represents primarily cholesterol which is associated with lipo- and chylomicrons.

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