MIGRATION OF MOUSE LYMPHOCYTES IN VITRO I. NORMAL LYMPH NODE LYMPHOCYTES

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Abstract
Normal unstimulated mouse lymph node lymphocytes (LNLs) migrated into filters in a gradient of normal mouse serum (NMS), heat-inactivated mouse serum (HI-MS), or zymosan-activated mouse serum (ZAS). Blind well chemotaxis chambers with 5-.mu.m pore size cellulose nitrate membranes were used. Migration was assessed both by the leading front technique and the mean aggregate number. A concentration of 2.5 .times. 106 LNL/ml or greater was needed to detect migration. LNL migration to 1% NMS was time dependent and was inhibited by cytochalasin B. Comparison of the migration patterns of LNL, neutrophils and macrophages revealed that all cell types were responsive to NMS. LNL responded as well to HI-MS as they did to NMS, neutrophils responded less well to HI-MS than to NMS, and macrophages did not respond to HI-MS. The LNL response to ZAS was significantly greater than the response to NMS or HI-MS and neutrophils and macrophages also responded strongly to ZAS. The migration of LNL from various mouse strains to NMS revealed that the LNL from different mouse strains possess varying degrees of motility. The factor in mouse serum which induced migration was not strain specific. The LNL from peripheral (inguinal, axillary and brachial) nodes demonstrated greater motility in response to NMS than mesenteric LNL. Using the checkerboard assay to discriminate chemotaxis from chemokinesis, mouse serum appeared to be solely chemokinetic when the leading front technique was used. Using the mean aggregate number technique, mouse serum was determined to be both chemokinetic and chemotactic for LNL. The method can be reliably used to study those factors which influence the motility of normal or altered lymphocyte populations.