Pepsinogen C and pepsin C. Further purification and amino acid composition

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Abstract
Pepsinogen C and pepsin C from the pig [stomach] have been further purified by chromatography on DEAE-cellulose and by exclusion chromatography and the specific activities (with haemoglobin substrate) found are higher than those previously reported. The final preparations are homogeneous on electrophoresis in starch gel at 3 pH values except for contamination with less than 4% of pepsinogen and pepsin respectively. Pepsinogen C, like pepsin C, contains no phosphate. The amino acid compositions show some marked differences from those of pepsinogen and pepsin especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleuclne. The molecular weights of the enzyme and zymogen, obtained from the amino acid compositions, are 41,400 and 36,000 respectively, similar to those of pepsinogen and pepsin.