Analysis of the Euglena gracilis chloroplast genome
- 1 November 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 149 (1) , 133-137
- https://doi.org/10.1016/0014-5793(82)81088-0
Abstract
The DNA fragment Eco‐I (4700 kilobasepairs) from the circular chloroplast genome of Euglena gracilis is shown to be transcribed in etioplasts at all stages of light‐induced plastid development and in fully differentiated chloroplasts. Major stable transcription products are mRNAs of 14S and 17S. Using a rabbit reticulocyte lysate translation system we can show that the fragment Eco‐I selects a mRNA which directs the synthesis of a M r 32 000–33 000 polypeptide. Eco‐I also hybridizes with a 330 basepair DNA probe cut from within the spinach chloroplast gene encoding the M r 32 000 thylakoid membrane protein of the photosystem II reaction center. We conclude that the fragment Eco‐I carries the corresponding Euglena gracilis chloroplast gene.Keywords
This publication has 24 references indexed in Scilit:
- Analysis of Euglena gracilis chloroplast DNAFEBS Letters, 1981
- Complexity and abundance of ribonucleic acid transcribed from restriction endonuclease fragments of Euglena chloroplast deoxyribonucleic acid during chloroplast developmentBiochemistry, 1981
- Preparation of Chloroplasts from Euglena Highly Active in Protein SynthesisPlant Physiology, 1980
- Synthesis of polypeptides of the chlorophyll—protein complexes in isolated chloroplasts of Euglena gracilisFEBS Letters, 1980
- Hybridization of EcoRI chloroplast DNA fragments of Euglena to pulse labeled RNA from different stages of chloroplast developmentBiochemical and Biophysical Research Communications, 1979
- Transcription program of the chloroplast genome of Euglena gracilis during chloroplast developmentProceedings of the National Academy of Sciences, 1979
- Large subunit ribulosebisphosphate carboxylase messenger RNA from Euglena chloroplasts.Proceedings of the National Academy of Sciences, 1976
- Changes in the expression of the chloroplast genome of Euglena gracilis during chloroplast developmentBiochemistry, 1976
- A measurement of the fraction of chloroplast DNA transcribed during chloroplast development in Euglena gracilisBiochemistry, 1976
- Isolation of Intact Chloroplasts from Euglena gracilis by Zonal CentrifugationPlant Physiology, 1971