THE PRODUCTION OF HYBRIDOMAS FROM THE GUT ASSOCIATED LYMPHOID-TISSUE OF TUMOR-BEARING RATS .1. MESENTERIC NODES AS A SOURCE OF IGG PRODUCING CELLS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 57  (2) , 358-364
Abstract
Rat .times. rat hybridomas secreting antibodies with tumor specificity were prepared using cells from the mesenteric nodes of rats bearing syngeneic sarcomata in their Peyer''s patches. The antibodies obtained, which embraced all of the major Ig classes, varied in cellular reactivity from the individually tumor specific to cross-reactive with both normal and tumor cells. Several IgA producing hybridomas were prepared using this protocol, but IgG secretors were obtained more frequently and they accounted for .apprx. 1/2 the specific hybridomas. Specific IgG producers also predominated when hydridomas were prepared from the mesenteric nodes of a rat immunized by injection of horseradish peroxidase into the Peyer''s patches. Comparison of these data with earlier results using spleen cells taken from rats that were either hyperimmunized with, or were bearing, the same tumors in the leg show that mesenteric nodes draining a tumor growing in the Peyer''s patches are a much better source of specific IgG producing B cells.