A Study of an Unusual Hemagglutinin in a Pooled Human Serum

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Abstract
Summary: “Routine” antibody screening test of the D-1 serum showed the presence of only the “incomplete” Rho antibody. Trypsin-treated O rh cells agglutinated very strongly in this serum. The “T-agglutinin” was not present in this serum. The agglutination tests before and after absorption of the serum with untreated and enzyme-treated Rh-positive and Rh-negative cells show that the agglutination of the trypsinized red blood cells in this serum is due to an agglutinating factor independent of the Rho antibody. This factor was found to be equally reactive at 0°C, 22°C, and at 37°C for 30 minutes, and absorbable, nondialyzable and thermolabile at 65°C for one hour, unlike the reversible agglutinin factor.