THE STIMULATION OF GENE RECOMBINATION IN ESCHERICHIA COLI

Abstract
The rate of formation of prototrophs between mutant strains of E. coli K-12 can be increased by direct irradiation of the cells, irradiation of the substrate in which the organisms are grown, treatment of the nutrient substrate with hydrogen peroxide, or growth in the presence of Na azide. Induced back mutation of the strains did not occur with the treatments used; thus the increase of prototrophs must be due to increased recombination.
Keywords