Detection of pyrrolidone carboxylic acid

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Abstract
Observations on ionophoresis and partition chromatography of pyrrolidone carboxylic acid (PCA) are reported. PCA is conveniently detected by the procedure of Rydon and Smith (1952). PCA was detected in, and partially isolated from, Italian rye grass that had been stored at -20[degree]. In extracts of fresh grass the amounts of PCA are negligible compared with those of other forms of "bound" amino acids. Methods hitherto used for detecting and determining PCA are reviewed and possible improvements suggested.