SYNTHETIC ACTIVITY IN MOUSE ORGANS MAINTAINED ON CHEMICALLY-DEFINED MEDIA.

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Abstract
Atrophic uteri taken from ovariectomized C57 B1 mice hypertrophy when maintained as organ cultures in an atmosphere of 5% CO2-95% O2 on a medium made up of 4 parts Waymouth''s-199 synthetic medium and 1 part agar. Cells of both the endometrium and myometrium respond. Net amounts of protein are synthesized by the explants; after 6 days of culture, the net increment of protein was 60% on the basal medium alone and 80% when insulin was included. An effect of insulin on the specific radioactivity of uterine protein was evident as early as 8 hours after the explants had been placed on the culture medium that contained leucine-1-C14. The differences between the control and insulin groups were progressively accentuated over the course of the first 5 days of culture, but were not always apparent after as long as 6 days.

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