Ovalbumin is synthesized in mouse cells transformed with the natural chicken ovalbumin gene.
- 1 January 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (1) , 244-248
- https://doi.org/10.1073/pnas.77.1.244
Abstract
The entire chicken ovalbumin gene, accompanied by genomic DNA sequences flanking both termini of the gene and 3 copies of the herpes simplex virus thymidine kinase gene, was cloned in plasmid pBR322. This recombinant plasmid was linearized and used to transform thymidine kinase-deficient mouse cells. Thymidine kinase-positive transformants were selected by their ability to grow in the hypoxanthine/aminopterin/thymidine (HAT) medium. The entire ovalbumin gene integrated into high MW DNA within all the transformants and retained its original sequence organization. In all of the transformants examined, a protein identified as chicken ovalbumin by immunoreactivity was detected within the cells. It is estimated that between 1000 and 100,000 molecules of chicken ovalbumin were produced per mouse cell in each of these transformants. The mouse cellular machinery can be utilized to accurately express genetic information encoded in a cloned gene from a different eukaryotic organism into its specific protein product.This publication has 20 references indexed in Scilit:
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