The survival, function, and hemolysis of human RBCs stored at 4°C in additive solution (AS‐1, AS‐3, or AS‐5) for 42 days and then biochemically modified, frozen, thawed, washed, and stored at 4°C in sodium chloride and glucose solution for 24 hours

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Abstract
BACKGROUND: A study was done to assess the quality of RBCs stored at 4°C in AS‐1, AS‐3, or AS‐5 for 42 days before biochemical modification and freezing.STUDY DESIGN AND METHODS: RBCs were stored at 4°C for 42 days in AS‐1, AS‐3, or AS‐5 and then biochemically modified with pyruvate, inosine, phosphate, and adenine solution (Rejuvesol), frozen with 40‐percent (wt/vol) glycerol, and stored at –80°C for at least 2 months. The RBCs were deglycerolized by the use of a cell washer (Haemonetics 115), and stored for 24 hours at 4°C in a 0.9‐percent sodium chloride and 0.2‐percent glucose solution before the autologous transfusion.RESULTS: The mean freeze‐thaw‐wash recovery process produced RBC recovery values of 85 percent, with the mean 24‐hour posttransfusion survival at 75 percent, and the mean index of therapeutic effectiveness at 64 percent for the RBCs stored at 4°C in AS‐1, AS‐3, or AS‐5 for 42 days before biochemical modification and freezing. All the units exhibited normal or slightly higher than normal 2,3 DPG levels after deglycerolization and postwash storage at 4°C for 24 hours.CONCLUSION: RBCs stored in AS‐1, AS‐3, or AS‐5 at 4°C for 42 days and then biochemically modified with pyruvate, inosine, phosphate, and adenine and glycerolized, frozen, washed, and stored at 4°C for 24 hours before autologous transfusion had acceptable in vitro and in vivo measurements.

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