Studies on Transformations of Hemophilus influenzae

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Abstract
Sedimentation and diffusion coefficients have been determined for the transforming activity and DNA of Hemophilus influenzae using P32-labeled DNA. The methods employed the Spinco fixed boundary separation cell for measurements of the sedimentation coefficient and the Northrop-Anson diffusion cell to determine the diffusion coefficient. There was a very close correlation between the amounts of DNA and of transforming activity. The sedimentation coefficient (s20O) for both biological activity and DNA was 27 and the diffusion coefficient (D20O) 1 x 10-8 cm2/sec. The molecular weight calculated was 16 million. There was no difference in the sedimentation coefficients for the two unlinked markers, streptomycin and erythromycin resistance, and the diffusion coefficients for single markers or the linked markers, streptomycin and cathomycin, were the same.

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