Changes in Adrenal Corticosterone Concentration in Rats: Method of Bio-assay for ACTH

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Abstract
A method is described for the bioassay of ACTH in 24-hr. hypophysectomized rats. It is based on the increase in adrenal corticosterone concentration resulting from the intravenous administration of ACTH. Maximum adrenal corticosterone concentrations are attained 5 min. after such an injection for the range of ACTH doses effective in this method. A rectilinear log-dose response relationship exists between the doses of 25-200 [mu]U ACTH/rats. This method compares favorably in sensitivity, specificity, precision (mean [gamma] = s/b = 0.191) and ease of performance with existing methods. Resting levels of ACTH, determined in 3-5 ml samples of rat or human plasma, are readily detectable.

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