Kinesin motion in the absence of external forces characterized by interference total internal reflection microscopy
- 15 August 2003
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review E
- Vol. 68 (2) , 021907
- https://doi.org/10.1103/physreve.68.021907
Abstract
We study the motion of the kinesin molecular motor along microtubules using interference total internal reflection microscopy. This technique achieves nanometer scale resolution together with a fast time response. We describe the first in vitro observation of kinesin stepping at high ATP concentration in the absence of an external load, where the 8-nm step can be clearly distinguished. The short-time resolution allows us to measure the time constant related to the relative motion of the bead-motor connection; we deduce the associated bead-motor elastic modulus.Keywords
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