Über die anaerobe Resynthese von Phosphokreatin nach der Reizung isolierter Froschmuskeln.

Publisher Website
Google Scholar
Abstract
Forty-five control experiments justify comparisons of free and creatine-bound phosphoric acid in stimulated and unstimulated right and left isolated gastrocnemii muscles of frogs. The graphical estimation of the initial inorganic phosphoric acid in the Fiske and Sub-barow method is replaced by a method based upon least squares. Tetanic stimulation leads to a diminution in phosphocreatine; increasing the time of stimulation increases its splitting. The observation of Eggleton and Eggleton that phosphocreatine so split is regenerated during relaxation in O is confirmed. Muscles stimulated for 12 sec. and allowed to relax 30 min. in purified N exhibit anaerobic regeneration of phosphocreatine by a reaction believed to be strongly endothermic. Lengthening the stimulation to 25 sec. prevents anaerobic resynthesis of phosphocreatine, although it is still observed under aerobic conditions of relaxation. Definite anaerobic phosphocreatine regeneration was demonstrated in 5 of 7 experiments in which the 12 sec. stimulation was preceded and followed by 30 min. rest periods in N. Pretreating the muscle with 0 for 30 min. before stimulation appears to preserve the phosphocreatine. Some observations on variations in lactacidogen are included.