A NEW METHOD FOR PERMANENT PREPARATIONS OF HEMATOPOIETIC-CELLS CULTURED IN METHYLCELLULOSE MEDIUM

  • 1 January 1982
    • journal article
    • research article
    • Vol. 10  (2) , 145-150
Abstract
To obtain permanent preparations for morphologic examination of human hemopoietic cells cultured in methylcellulose medium, a new method designated the membrane filtration technique was devised. Permanent preparations of erythroid colonies, bursts and leukemic colonies, which have been grown mainly in methylcellulose medium, are illustrated. The stages of differentiation of cells within these colonies can be easily recognized by means of Wright-Giemsa staining.