Separation of Soybean Whey into Fractions with Different Biological Activities for Chicks and Rats

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Abstract
Using a batch fractionation procedure on DEAE cellulose, 4 fractions of soybean whey were prepared and studied for biological activity with chicks and rats. All fractions prepared had trypsin inhibitor activity but hemagglutinin activity and intraperitoneal toxicity for rats was concentrated in one fraction. The fractions differed in relative stimulation of the pancreas of chicks to enlarge and the gallbladder to contract. Differences were also noted among the fractions in their effects on chick growth, fat absorption and dietary metabolizable energy value. Many of the effects caused by the soybean fractions appeared to be related to several components rather than to one specific fraction.