Effects of prostaglandins E1, E2, and F2α on the growth of leukaemia cells in culture

Open Access
Publisher Website
Google Scholar
Abstract
Prostaglandins E1 E2, and F2α (PGE1; PGE2, and PGF2α) were shown to inhibit the growth of mouse leukaemia lymphoblasts L5178Y in culture. The effects of PGE! and PGE2 were greater than that of PGF2α. PGE1 and PGE2, at the concentration of 100 μg per ml showed significant inhibitory effects on the rates of incorporation of tritiated thymidine, uridine and leucine. At concentrations of 50 and 25 μg per ml, there was significant inhibition of thymidine and uridine incorporation, but not of leucine. PGF2a showed significant inhibition of thymidine and uridine incorporation but not leucine incorporation, in all 3 concentrations studied (100, 50, and 25 μg/ml). The ability of the cells to form colonies in soft agar was significantly inhibited by PGE1 and PGE2 at concentrations as low as 1·8 μg/ml. For F2α, however, a concentration as high as 56 μg/ml was required to show inhibitory effect, but at t1·8 μg ml it was found to be stimulatory.