On the modulating effects of temperature, albumin, pH and calcium on the free fractions of phenobarbitone and phenytoin
- 1 September 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 38 (9) , 643-646
- https://doi.org/10.1111/j.2042-7158.1986.tb03102.x
Abstract
The effects of temperature, albumin, pH and Ca2+ on the binding of phenobarbitone and phenytoin to human serum albumin in buffer have been investigated using equilibrium dialysis. The free fractions of both anticonvulsants were much increased by raising the temperature. Lower free fractions were observed by increasing the albumin concentration from 5–8 g litre−1 and by raising pH from 6 to 9. No significant effect on the free fractions was observed by changing (at pH 7.4) the Ca2+ concentration from 0 to 5 mM. The observed differences in free fractions at 37 °C., as determined in phosphate, borate and Krebs-Ringer buffer at pH 7.4, indicate that great care is needed in the choice of dialysis fluid for dialysis of clinical samples.This publication has 10 references indexed in Scilit:
- Binding of theophylline in human serum determined by ultrafiltration and equilibrium dialysis.British Journal of Clinical Pharmacology, 1983
- Plasma Protein Binding of Disopyramide by Equilibrium Dialysis and UltrafiltrationTherapeutic Drug Monitoring, 1983
- Factors influencing theophylline serum protein bindingClinical Pharmacology & Therapeutics, 1982
- Altered Drug Binding to Serum Proteins in Pregnant Women: Therapeutic RelevanceJournal of the Royal Society of Medicine, 1981
- The role of albumin conformation in the binding of diazepam to human serum albuminBiochimica et Biophysica Acta (BBA) - Protein Structure, 1980
- Serum protein binding of drugs during and after pregnancy in humansClinical Pharmacology & Therapeutics, 1980
- The effect of albumin conformation on the binding of warfarin to human serum albumin. The dependence of the binding of warfarin to human serum albumin on the hydrogen, calcium, and chloride ion concentrations as studied by circular dichroism, fluorescence, and equilibrium dialysis.Journal of Biological Chemistry, 1980
- Displacement of phenytoin from plasma binding sites by salicylateClinical Pharmacology & Therapeutics, 1980
- Plasma Protein Binding and Therapeutic Drug MonitoringTherapeutic Drug Monitoring, 1979
- EFFECT OF PH ON THE BINDING OF THEOPHYLLINE TO SERUM-PROTEINSPublished by Elsevier ,1979