Influence of Various Agents on Mast Cells Isolated from Rat Peritoneal Fluid.

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Abstract
In vitro studies on mast cells isolated from rat peritoneal fluid revealed: Metachromatic granules were partially removed by testicular hyaluronidase, but when rats were pretreated systemically with Escherichia coli endotoxin, 2 mg intravenously, this effect of hyaluronidase was much more extensive. E. coli endotoxin, 0.5%, did not influence the mast cells in vitro. Metachromatic staining of the cells was prevented, and if previously stained the color was abolished, by compound 48/80 (formaldehyde condensation product of p-methoxy-N-methyl phenethyl amine) or protamine sulfate. These agents and serotonin, 0.5%, neither degranulated nor disrupted the cells, but they produced cell clumping. Heparin, over 0.005% caused cell disintegration, whereas 0.5% hyaluronic acid or histamine did not appear to affect the cells adversely. Hyaluronic acid seemed to protect them to some degree against degranulation.