The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing.
Open Access
- 1 April 1990
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 10 (4) , 1805-1810
- https://doi.org/10.1128/mcb.10.4.1805
Abstract
Polymerase chain reaction analysis was used to identify aberrant splicing of the simian virus 40 small-t intron present in pRSVcat. We examined factors governing the selection and relative use of aberrant 5' splice sites derived from the chloramphenicol acetyltransferase-coding region. Our results indicated that transcripts from virtually any cDNA positioned upstream of the small-t intron could contain alternative 5' splice sites and therefore be subject to deletions within the protein-coding region.This publication has 41 references indexed in Scilit:
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