THE INDEPENDENT ESTIMATION OF 11-DESOXYCORTISOL AND CORTISOL IN A SINGLE PLASMA SAMPLE*
- 1 August 1961
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 21 (8) , 943-954
- https://doi.org/10.1210/jcem-21-8-943
Abstract
A method for the determination of cortisol and 11-desoxycortisol in a single small sample of blood plasma is described. This column chromatographic method employs, successively, Florisil and silica gel columns for purification and separation of cortisol and 11-desoxycortisol. After separation of the steroids, each is measured quantitatively by use of the Porter-Silber reaction. Values were determined for cortisol and 11-desoxycortisol in the early morning blood plasma of normal subjects. The application of the method in conjunction with the SU-4885 test is illustrated by presentation of the results obtained in 2 normal subjects and in 2 patients with Cushing's syndrome due to adrenal perplasia.Keywords
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