PHOSPHONOLIPIDS: XIV. DETECTION AND IDENTIFICATION OF GLYCEROLPHOSPHONOLIPIDS IN THE PRESENCE OF THEIR PHOSPHOLIPID ANALOGUES BY PAPER CHROMATOGRAPHY OF THEIR SAPONIFICATION PRODUCTS
- 1 December 1967
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 45 (12) , 1783-1787
- https://doi.org/10.1139/o67-211
Abstract
A procedure for the detection and identification of α-glycerolphosphonolipids in the presence of their phosphoric acid analogues, viz. α-lecithins and α-cephalins, is described. It combines the saponification of these compounds by a modified Dawson procedure with the separation and identification of the saponification products by descending paper chromatography with n-butanol, glacial acetic acid, and water (5:3:1, v/v/v), and staining of the chromatograms with ninhydrin and molybdate sprays.The relative mobilities of the saponification products, viz, α-glycerol-(2-trimethylammoniumethyl)phosphonate, α-glyceryl-(2-aminoethyl)phosphonate, α-glycerolphosphorylcholine, and α-glycerylphosphorylethanolamine with respect to each other and to α-glycerophosphoric acid have been determined.This publication has 14 references indexed in Scilit:
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