The three‐dimensional structure of thymidine kinase from Herpes simplex virus type 1
- 17 July 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 368 (2) , 289-292
- https://doi.org/10.1016/0014-5793(95)00680-8
Abstract
Recombinant thymidine kinase from Herpes simplex virus type 1 (ATP:thymidine 5′-phosphotransferase; EC 2.7.1.21), an enzyme of therapeutic importance, was purified and crystallized in an N-terminally truncated but still fully active form. The three-dimensional structure was solved by X-ray diffraction analysis at 3.0 Å resolution using isomorphous replacement. The chain fold is presented together with the bound substrates thymidine and ATP. Three chain segments at the surface could not be located. The chain fold, the location of the substrates and presumbly also the catalytic mechanism resemble the well-known adenylate kinasesKeywords
This publication has 17 references indexed in Scilit:
- High‐resolution structures of adenylate kinase from yeast ligated with inhibitor Ap5A, showing the pathway of phosphoryl transferProtein Science, 1995
- Site‐Directed Mutagenesis Clarifies the Substrate Position within the Three‐Dimensional Model of the Active Site of Herpes Simplex Virus Type‐1 Thymidine KinaseEuropean Journal of Biochemistry, 1994
- Site-Directed Mutagenesis Clarifies the Substrate Position within the Three-Dimensional Model of the Active Site of Herpes Simplex Virus Type-1 Thymidine KinaseEuropean Journal of Biochemistry, 1994
- Site-directed mutagenesis in the active site of the herpes simplex virus type 1 thymidine kinase geneVirus Genes, 1993
- Structure of the complex between adenylate kinase from Escherichia coli and the inhibitor Ap5A refined at 1.9 Å resolutionJournal of Molecular Biology, 1992
- Binding of nucleotides by proteinsCurrent Opinion in Structural Biology, 1992
- Herpesviral Deoxythymidine Kinases Contain a Site Analogous to the Phosphoryl-binding Arginine-rich Region of Porcine Adenylate Kinase; Comparison of Secondary Structure Predictions and ConservationJournal of General Virology, 1990
- Purification and crystallization of thymidine kinase from herpes simplex virus type 1Journal of Molecular Biology, 1988
- The glycine‐rich loop of adenylate kinase forms a giant anion holeFEBS Letters, 1986
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976