VACCINATION OF BALB/C MICE AGAINST BRUGIA-MALAYI AND B-PAHANGI WITH LARVAE ATTENUATED BY GAMMA-IRRADIATION

  • 1 January 1984
    • journal article
    • research article
    • Vol. 54  (4) , 177-181
Abstract
The vaccination with radiation-attenuated infective larvae of B. malayi and B. pahangi was attempted and evaluated in BALB/c mice. Two weeks after i.p. infection with 100 3rd-stage larvae, the worms of both species of Brugia were recovered in the peritoneal cavity of BALB/c mice. The average recovery was more than 20% in both Brugia infections. Groups of 10 mice were vaccinated subsequently 3 times with 100 3rd-stage larvae of B. malayi or B. pahangi attenuated by 20 krad .gamma. irradiation and challenged with the homologous species. Vaccinated mice showed a 95.5% reduction in recovered worms in the challenge infection with B. malayi as compared to the infection in non-vaccinated controls, and a 93.8% reduction in the B. pahangi group.