Bacteriophages from Sewage Specific for Fluorescent Phytopathogenic Pseudomonads

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Abstract
Fifty-six bacteriophages were isolated on Pseudomonas syringae pathovar glycinea from raw sewage obtained from 4 sources in Riverside and San Bernardino counties in California [USA]. Seven selected phages characterized further were specific for the fluorescent phytopathogenic pseudomonads, particularly for the P. syringae group. Not all pathovars of P. syringae were susceptible to these phages. One of these phages was specific to pathovar glycinea race 4. EM characterization of 6 of the 7 selected phages revealed 4 distinct morphologies. All of the phages examined contained double-stranded DNA. The phages were tested for generalized transducing ability of several different chromosomal genes. No stable recombinants were recovered regardless of the conditions used in transduction experiments.