A Simple Automated Injection Technique for the High-Pressure Liquid Chromatographic Determination of Plasma Retinol
- 1 September 1983
- journal article
- research article
- Published by SAGE Publications in Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
- Vol. 20 (5) , 308-311
- https://doi.org/10.1177/000456328302000511
Abstract
We describe a rapid automated method for determination of retinol in 100 μl plasma. A lipid extract of plasma is analysed by reverse phase high pressure liquid chromatography using methanol-water as eluent. Automation of injection is achieved by adaptation of a Technicon AutoAnalyzer Sampler 2 coupled with a Micromedic dilution system and a pneumatically actuated loop injector. Twenty patients' specimens can be analysed in 4 hours, including extraction time, standards, and quality controls, with one extract injected every 4 minutes.This publication has 7 references indexed in Scilit:
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