NUCLEOTIDE-SEQUENCE STUDIES OF POLYOMA DNA - HPAII 3-5 JUNCTION TO HPA II4-HAEIII18 JUNCTION, ENCODING ORIGIN OF DNA-REPLICATION AND 5' END OF EARLY REGION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 253  (18) , 6561-6567
Abstract
The nucleotide sequence of polyoma DNA, from near the Hpa II 3/5 junction to the Hpa II 4/Hae III 18 junction was determined by the chemical method of Maxam and Gilbert. The sequence contains 878 base pairs, including the origin of DNA replication and the region known to encode the hr-t function. The region corresponding to the origin of DNA replication contains several short repeated sequences and palindromes. There is a 30-base-pair region with striking similarity to the corresponding region of SV-40, and, as in SV-40, a portion of that sequence is capable of forming a stable hairpin loop. In the region encoding the hr-t function, there is apparently a single open reading frame extending from position 188 to the Hpa III 4/Hae III 18 junction. The potential translation product of this open frame begins with an initiation codon starting at position 188, and the first 5 amino acids of this product are Met-Asp-Arg-Val-Leu. This sequence is similar to the NH2-terminal 5 amino acids of SV-40 small t-antigen known from nucleotide and amino acid sequencing to be Met-Asp-Lys-Val-Leu.