Cell‐wall lipopolysaccharide of the urinary‐tract‐infective Escherichia coli 04:K12:H−
- 1 December 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 137 (1-2) , 163-171
- https://doi.org/10.1111/j.1432-1033.1983.tb07810.x
Abstract
The O-specific polysaccharide moiety (04 polysaccharide) of the 04 antigen (lipopolysaccharide) from E. coli 04:K12:H- was isolated in pure form by degradation of the lipopolysaccharide and chromatography on Sephadex G-50. The primary structure of the 04 polysaccharide was elucidated by composition, Smith degradation. NMR spectroscopy, methylation and oligosaccharide analysis. Oligosaccharides were obtained by deaminating fragmentation of partially and completely de-N-acetylated polysaccharides. The polysaccharide consists of repeating units of a pentasaccharide joined in the polymer through .alpha.-1,4-linkages. The 04 polysaccharide has a mean MW of 13,800 daltons and consists of the core oligosaccharide and .apprx. 14 pentasaccharide repeating units.This publication has 25 references indexed in Scilit:
- Structure of the 2-Keto-3-deoxy-d-manno-octonic-acid-Containing Capsular Polysaccharide (K12 Antigen) of the Urinary-Tract-Infective Escherichia coli O4:K12:H-European Journal of Biochemistry, 1983
- Somatic Antigens of Pseudomonas aeruginosaEuropean Journal of Biochemistry, 1982
- Structures of Fifteen Oligosaccharides Isolated from New‐Born MeconiumEuropean Journal of Biochemistry, 1981
- The application of 500‐MHz 1H‐NMR spectroscopy for the structure elucidation of N‐acetyllactosamine type asparagine‐bound carbohydrate chains of glycoproteinsFEBS Letters, 1980
- Enzymic synthesis and carbon-13 nuclear magnetic resonance conformational studies of disaccharides containing .beta.-D-galactopyranosyl and .beta.-D-[1-13C]galactopyranosyl residuesBiochemistry, 1980
- Carbon-13 nuclear magnetic resonance spectroscopy of [1-13C] enriched monosaccharides. Signal assignments and orientational dependence of geminal and vicinal carbon-carbon and carbon-hydrogen spin-spin coupling constantsJournal of the American Chemical Society, 1976
- A study of 13CH coupling constants in hexopyranosesJournal of the Chemical Society, Perkin Transactions 2, 1974
- Determination of the Anomeric Configuration of Sugar Residues in Acetylated Oligo- and Polysaccharides by Oxidation with Chromium Trioxide in Acetic Acid.Acta Chemica Scandinavica, 1972
- RAPID DETERMINATION OF MOLECULAR WEIGHTS OF PEPTIDES AND PROTEINS*Annals of the New York Academy of Sciences, 1960
- The Chromic Acid Oxidation of Isopropyl Alcohol in 86.5% Acetic Acid Solution. The Chemistry of the Chlorochromate IonJournal of the American Chemical Society, 1952