Evidence for the Presence of Oncoplacental SP1-Like Protein in Normal Nonpregnant Serum*
- 1 July 1985
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 61 (1) , 188-191
- https://doi.org/10.1210/jcem-61-1-188
Abstract
To seek the pregnancy-specific β1-glycoprotein (SP1) in nonpregnant serum, normal human serum was applied to immunoadsorbent containing monoclonal anti-SP1 antibodies. SP1 eluted with 8 M urea was further analyzed by sodium dodecyl sulfate-gel electrophoresis and immunoblotting. A SP1–positive band with the same electrophoretic mobility as purified placental SP1 was found. The results suggest that serum from normal nonpregnant subjects contains material closely related to the placental protein SP1. The mean serum concentrations of SPi were similar in men and women, ranging from 1.1–3.4 ng/ml.Keywords
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