Accumulation of Ribonucleoprotein Particles in a Relaxed Mutant of Escherichia coli

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Abstract
The synthesis of ribonucleic-acid during amino-acid deprivation of a "relaxed" mutant was investigated. Aspects of the stability of the macromolecular ribonucleic-acid (RNA) were studied, and standard conditions were established to allow maximal recovery of the larger RNA''s (i.e., 16S and 23S). These RNA''s, representing 75% of the total RNA produced during starvation, were present in particles with nominal S-values of 20, 30 and 43. The particles are extremely sensitive to nuclease action, being completely destroyed in the presence of 2 [mu]g/ml of pancreatic ribonuclease at 15 C for 30 min. The particles containing the bulk of the RNA were shown to be ribonucleoprotein, consisting of 26 to 28% protein by weight. It was shown that no mature 70S ribosomes were formed during the accumulation of the lighter, protein-deficient particles.

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