Activation of caspase-12, an endoplasmic reticulum resident caspase, after permanent focal ischemia in rat
- 1 February 2003
- journal article
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 14 (2) , 183-186
- https://doi.org/10.1097/00001756-200302100-00004
Abstract
The endoplasmic reticulum (ER) is emerging as a contributory component of cell death after ischemia. Since caspase-12 has been localized to the ER and is a novel signal for apoptosis, we examined the message levels and protein expression of caspase-12 after cerebral ischemia in vivo. Animals underwent permanent middle cerebral artery occlusion (MCAO) and were sacrificed 24 h after ischemia. Protein analysis revealed a significant increase in caspase-12 and a corresponding up-regulation of caspase-12 mRNA in the ischemia group compared with that in the sham group. Immunohistochemical analysis revealed diffuse positive immunostaining of caspase-12 throughout the striatum and cerebral cortex in animals that underwent ischemia, with more intense caspase-12 immunostaining in the striatum than in the cortex after ischemia. These results demonstrate that cerebral ischemia initiates an ER-based stress response that results in the transcriptional up-regulation and corresponding increased expression of caspase-12 protein, and may provide a new area for therapeutic intervention to ameliorate outcomes following stroke.Keywords
This publication has 19 references indexed in Scilit:
- Coupling endoplasmic reticulum stress to the cell death program: role of the ER chaperone GRP78FEBS Letters, 2002
- Formation of noncanonical high molecular weight caspase-3 and -6 complexes and activation of caspase-12 during serum starvation induced apoptosis in AKR-2B mouse fibroblastsCell Death & Differentiation, 2002
- Caspase-9 Inhibition after Focal Cerebral Ischemia Improves Outcome following Reversible Focal IschemiaMetabolic Brain Disease, 2002
- Aβ(1-42) and aluminum induce stress in the endoplasmic reticulum in rabbit hippocampus, involving nuclear translocation of gadd 153 and NF-κBMolecular Brain Research, 2001
- Coupling Endoplasmic Reticulum Stress to the Cell Death ProgramJournal of Biological Chemistry, 2001
- Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-βNature, 2000
- Involvement of Caspases in Proteolytic Cleavage of Alzheimer’s Amyloid-β Precursor Protein and Amyloidogenic Aβ Peptide FormationCell, 1999
- Ordering the Cytochrome c–initiated Caspase Cascade: Hierarchical Activation of Caspases-2, -3, -6, -7, -8, and -10 in a Caspase-9–dependent MannerThe Journal of cell biology, 1999
- Viability thresholds and the penumbra of focal ischemiaAnnals of Neurology, 1994
- Calcium‐activated neutral protease and its endogenous inhibitor Activation at the cell membrane and biological functionFEBS Letters, 1987