High resolution particle sizing using the combination of time-of-flight and light-scattering measurements

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Abstract
To achieve a high precision of sizing of particles in flow we investigated the combination of light scattering and time-of-flight (TOF) measurements using two laser beams different in wavelength and spot size. Lightscattering vs TOF size measurements done on polysterene beads (0.481–5 µm) demonstrate convincingly the multivalue nature of light-scattering data as predicted by Lorenz–Mie theory. Using the size information by TOF measurements the angular regions for high resolution sizing by light-scattering measurements are predictable and could be selected during the experiment. Measurements done on a mixture of spheres and biological cells (thymocyte nuclei, fixed lymphocytes) show that combining TOF and light-scattering dual-parameter measurements allow excellent discrimination and sizing of cells.