PREPARATION OF MAJOR PLASMA FRACTIONS BY SOLID-PHASE POLYELECTROLYTES

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 92  (2) , 194-210
Abstract
The solid-phase ethylene/maleic anhydride PE [polyelectrolyte] E-5 and E-100 were used to develop a flexible integrated system of batch methods for the fractionation of albumin and .gamma.-globulin as well as the clinically important coagulation factors. Each method for the isolation and purification of the fractions or a modification of it could also be used independently or could be added onto another method or methods. Each fraction was prepared in the following 3 steps: adsorption on solid-phase PE, elution from the PE and concentration by ultrafiltration or by use of PEG [polyethylene glycol] or both. Since the methods required no organic solvent but relied primarily on electrostatic bonding, they were essentially nondenaturing. Solid-liquid phase separation was easily accomplished by centrifugation or filtration. The yield and purity of each fraction was as follows: coagulation factors II, VII, IX and X concentrates 63% and 200-fold purified; AHF [antihemophilic factor] 43% and 52-fold; vWF [von Willebrand factor] 43% and 71-fold; PPF [plasma protein fraction], as albumin, 93% yield and 92% pure; albumin 92% yield and 98.5% pure; and .alpha.-globulin 92% yield and 97.5% pure. During the preparation of .alpha.-globulin the E-100 also tended to adsorb and remove the HBsAg [hepatitis B surface antigen] and infectivity when present.