Phorbol ester modulates serotonin receptor‐mediated increases in inositol phosphate production and calcium mobilization in cultured rat vascular smooth muscle cells

Abstract
The effect of 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) on serotonin‐induced inositol phosphate (IP) accumulation and intracellular free Ca2+ concentrations ([Ca2+]i) was investigated in cultured rat vascular smooth muscle cells. Pretreatment with TPA had no effect on basal levels of both IP production and [Ca2+]i, whereas it significantly attenuated serotonin‐induced increases in both IP production and [Ca2+]i. These data suggest that protein kinase C is involved in the negative feedback control of serotonin‐induced rises in both IP production and [Ca2+]i.

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