The isolation of N-phosphoryl-lombricine from earthworms

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Abstract
A procedure for isolating analytically pure N-phosphoryl-lombricine (magnesium salt) from perchloric acid extracts of frozen earthworms is described. The procedure employs anion- and cation-exchange resins for the major purification steps and produces yields of [image]-phosphoryl-lombricine in excess of 70% of that present in the original extracts.