Confocal laser-induced absorption microscope
- 1 June 1992
- journal article
- Published by Optica Publishing Group in Journal of the Optical Society of America A
- Vol. 9 (6) , 932-936
- https://doi.org/10.1364/josaa.9.000932
Abstract
A new laser scanning microscope is proposed for imaging a three-dimensional absorption structure of a sample. The microscope is based on a confocal system built with excitation and absorption-monitoring lasers. The excitation laser produces excited molecules, and the absorption-monitoring laser probes their transient absorption. A three-dimensional optical transfer function of the microscope is theoretically derived to demonstrate a tomographic imaging capability. The spatial frequency cutoff is confirmed to be independent of the detector size. An experimental result is shown to verify the depth discrimination property.Keywords
This publication has 11 references indexed in Scilit:
- Three-Dimensional Space- and Time-Resolved Fluorescence SpectroscopyApplied Spectroscopy, 1991
- Three-dimensional optical-transfer-function analysis for a laser-scan fluorescence microscope with an extended detectorJournal of the Optical Society of America A, 1991
- Three-dimensional imaging in a microscopeJournal of the Optical Society of America A, 1989
- Optical sectioning in confocal fluorescent microscopesJournal of Microscopy, 1989
- Size of the detector in confocal imaging systemsOptics Letters, 1987
- Depth response of confocal optical microscopesOptics Letters, 1986
- Three-dimensional imaging by a microscopeJournal of the Optical Society of America A, 1985
- Depth Transfer by an Imaging SystemOptica Acta: International Journal of Optics, 1984
- Three-dimensional architecture of a polytene nucleusNature, 1983