Studies on sulphatases. 4. Arylsulphatase and β-glucuronidase concentrates from limpets
- 1 September 1953
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 55 (2) , 315-320
- https://doi.org/10.1042/bj0550315
Abstract
Acetone-dried prepns. of limpet viscera were fractionated to give stable powders containing both arylsulphatase and beta-glucuronidase. Irreversible inactivation of the beta-glucuronidase at pH 2.25 yielded a stable arylsulphatase prepn. free from beta-glucuronidase. Total inhibition of arylsulphatase by 0.2 [image] phosphate allows the use of the crude viscera concentrates as beta-glucuronidase prepns. free from arylsulphatase. The inhibition of arylsulphatase by urine can be overcome by precipitating phosphate and sulphate ions with barium chloride.This publication has 8 references indexed in Scilit:
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